F9512
Anti-Human IgG (Fc specific)−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat, Anti Human Igg Fitc
biological source
goat
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunofluorescence: 1:64-1:128 using Hep2 cells
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG.
Immunogen
Purified human IgG Fc fragment
Application
Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in:
- immunofluorescence
- immunohistochemistry
- surface labeling experiments
Biochem/physiol Actions
Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples
Provides reduced background with mouse or rat samples
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Other Notes
No cross-reaction with mouse and rat IgG.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Regulatory Information
常规特殊物品
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Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-kappaB signaling pathway
Wang L, et al.
Oncology Letters, 12(6), 4411-4418 (2016)
Sebastian B Riese et al.
Journal of immunology (Baltimore, Md. : 1950), 192(4), 1862-1869 (2014-01-17)
Selectins are a family of adhesion receptors designed for efficient leukocyte tethering to the endothelium under shear. As a key property to resist premature bond disruption, selectin adhesiveness is enhanced by tensile forces that promote the conversion of a bent
Roshan James et al.
Cancer medicine, 1(2), 114-127 (2013-01-24)
Nimotuzumab, an anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibody, has been used extensively in many solid tumors and confers significant survival advantage. The antibody has limited skin toxicity and is generally well tolerated. Similar to other anti-EGFR therapies, patients may
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