FLE250
Firefly Lantern Extract
for ATP determination
Synonym(s):
FLE
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About This Item
NACRES:
NA.81
UNSPSC Code:
12352202
Product Name
Firefly Lantern Extract, for ATP determination
form
solution
packaging
pkg of 250 mg dried lanterns
technique(s)
toxicology assay: suitable
storage temp.
−20°C
Quality Level
Related Categories
Biochem/physiol Actions
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
Application
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
General description
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
Other Notes
Crude luciferin and luciferase
Preparation Note
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
动植物来源生物产品
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Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions
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