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G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

Name: Granulocyte-Macrophage Colony-Stimulating Factor from mouse
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

GM-CSF

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00241918

Biological source:

mouse

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

~15 kDa (125 amino acids including N-terminal methionine)

Impurities:

≤1 EU/mg

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Product Name

Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)

quality

endotoxin tested

mol wt

~15 kDa (125 amino acids including N-terminal methionine)

packaging

pkg of 5 and 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg

color

white

solubility

water: soluble, clear, colorless

UniProt accession no.

P01587

storage temp.

−20°C

Gene Information

mouse ... CSF2(12981), Csf2(12981)

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Analysis Note

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

常规特殊物品

This item has

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Certificates of Analysis (COA)

Lot/Batch Number

0000480315

0000408662

0000274734

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Mechanism of activation of the GM-CSF, IL-3, and IL-5 family of receptors.

M A Guthridge et al.

Stem cells (Dayton, Ohio), 16(5), 301-313 (1998-10-10)

The process of ligand binding leading to receptor activation is an ordered and sequential one. High-affinity binding of GM-CSF, interleukin 3 (IL-3), and IL-5 to their receptors induces a number of key events at the cell surface and within the

The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus.

M H Heim

Journal of receptor and signal transduction research, 19(1-4), 75-120 (1999-03-11)

The Jak-STAT pathway was originally discovered through the study of interferon induced intracellular signal transduction. Meanwhile, a large number of cytokines, hormones and growth factors have been found to activate Jaks and STATs. Jaks (Janus Kinases) are a unique class

Differential contributions of microglial and neuronal IKKβ to synaptic plasticity and associative learning in alert behaving mice.

Vasiliki Kyrargyri et al.

Glia, 63(4), 549-566 (2014-10-10)

Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion

Cytokines: principles and prospects.

A Kelso

Immunology and cell biology, 76(4), 300-317 (1998-09-02)

Cytokines participate in the induction and effector phases of all immune and inflammatory responses. They are therefore obvious tools and targets for strategies designed to promote, inhibit or redirect these responses. However, the complexity of the cytokine network has hindered

Specificity of signaling by hematopoietic cytokine receptors: instructive versus permissive effects.

R C Skoda

Journal of receptor and signal transduction research, 19(1-4), 741-772 (1999-03-11)

The helical cytokines constitute a family of proteins with a common three-dimensional structure. They exert a wide variety of biological effects with a preference for the hematopoietic system. The effects of helical cytokines are mediated by cell surface receptors, which

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Global Trade Item Number

SKU	GTIN
G0282-50UG	04061833623411
G0282-5UG	04061833623435

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