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G0751

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

≥300,000 units/g solid

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Properties

type

Type H-1

Quality Level

form

partially purified powder

specific activity

≥300,000 units/g solid

secondary activity

≥10,000 units/g solid sulfatase

storage temp.

−20°C

Description

General description

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

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pictograms

Health hazard

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

Regulatory Information

低风险生物材料

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Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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Global Trade Item Number

SKU	GTIN
G0751-500KU	04061835556953
G0751-100KU	04061833624142
G0751-2MU	04061833624166
G0751-1MU	04061833624159