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G1401

Sigma-Aldrich

Glial Cell Line-derived Neurotrophic Factor from rat

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

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Synonym(s):
ATF, Astrocyte-derived trophic factor, GDNF
MDL number:
MFCD01093875
NACRES:
NA.32

biological source

rat

Quality Level

200

recombinant

expressed in baculovirus infected Sf21 cells

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.10-10 ng/mL

mol wt

~30 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

Q07731

storage temp.

−20°C

Gene Information

rat ... Gdnf(25453)

Related Categories

Biochem/physiol Actions

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Physical form

Lyophilized from 50 μL of a 0.2 um filtered solution in PBS, pH 7.4 with 50 ug BSA per 1 ug as a carrier protein

Analysis Note

The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Caroline Perner et al.
STAR protocols, 2(1), 100333-100333 (2021-02-23)
In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide
Roche C de Guzman et al.
Journal of microencapsulation, 25(7), 487-498 (2009-02-25)
Controlled expression of glial cell line derived neurotrophic factor (Gdnf) can be integrated in the development of a system for repair of injured peripheral nerves. This delivery strategy was demonstrated via inducible Gdnf from microencapsulated cells in barium alginate. The
Weidong Xiao et al.
Molecular neurobiology, 50(2), 274-289 (2014-06-01)
Acute intestinal ischemia reperfusion (IR) injury is often associated with intestinal epithelial barrier (IEB) dysfunction. Enteric glial cells (EGCs) play an essential role in maintaining the integrity of IEB functions. However, the precise mechanism of EGCs under IR stimulation remains
Clive Bate et al.
Molecular neurodegeneration, 3, 1-1 (2008-01-09)
The early stages of Alzheimer's disease (AD) are closely associated with the production of the Abeta1-42 peptide, loss of synapses and gradual cognitive decline. Since some epidemiological studies showed that EGb 761, an extract from the leaves of the Ginkgo
Michela Morano et al.
International journal of nanomedicine, 9, 5289-5306 (2014-12-09)
Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits
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