Glial Cell Line-derived Neurotrophic Factor human

Recombinant human Glial Cell Line‑Derived Neurotrophic Factor (GDNF) is expressed in E. coli and supplied as a lyophilized powder. GDNF is a member of the GDNF family of ligands and is used in in vitro research involving neuronal cell culture.

GDNF has been used in neuronal and stem cell culture research applications to support cell survival and differentiation in vitro. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

GDNF is a neurotrophic factor that mediates cellular responses through GDNF family receptor signaling complexes and has been extensively studied in neuronal cell models in vitro. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Mature human GDNF, a disulfide-linked homo­dimeric glycoprotein, is predicted to contain two 15 kDa polypeptide chains.

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.