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G211000

DMT-2′O-TBDMS-rG(ib) Phosphoramidite

Name: DMT-2′O-TBDMS-rG(ib) Phosphoramidite
Brand: SIGMA

Synonym(s):

N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]

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About This Item

Empirical Formula (Hill Notation):

C₅₀H₆₈N₇O₉PSi

CAS Number:

147201-04-5

Molecular Weight:

970.18

NACRES:

NA.51

PubChem Substance ID:

329799851

UNSPSC Code:

12352200

MDL number:

MFCD00080299

Assay:

≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)

Biological source:

non-animal source (no BSE/TSE risk)

Form:

powder

Storage temp.:

−20°C

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Properties

biological source

non-animal source (no BSE/TSE risk)

Quality Segment

300, 200

product line

Proligo Reagents

assay

≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)

form

powder

technique(s)

oligo synthesis: suitable

impurities

≤0.3 wt. % water content (Karl Fischer), ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR), ≤0.5% single Impurity (reversed phase HPLC), ≤3 wt. % residual Solvent content

color

white to off-white

λ

conforms (UV/VIS Identity)

suitability

conforms to structure for H-NMR, conforms to structure for LC-MS

nucleoside profile

base: guanosine
base protecting group: isobutyryl
2' protecting group: TBDMS
5' protecting group: DMT
deprotection: standard

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)C(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6

InChI

1S/C50H68N7O9PSi/c1-32(2)45(58)54-48-53-44-41(46(59)55-48)52-31-56(44)47-43(66-68(12,13)49(7,8)9)42(65-67(63-29-17-28-51)57(33(3)4)34(5)6)40(64-47)30-62-50(35-18-15-14-16-19-35,36-20-24-38(60-10)25-21-36)37-22-26-39(61-11)27-23-37/h14-16,18-27,31-34,40,42-43,47H,17,29-30H2,1-13H3,(H2,53,54,55,58,59)/t40-,42-,43-,47-,67?/m1/s1

InChI key

PGJKESPHANLWME-FTZVBZPOSA-N

Description

General description

DMT-2′O-TBDMS-rG(ib) Phosphoramidite belongs to the group of 2′O-TBDMS RNA Phosphoramidites.

Application

RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence-specific RNA oligonucleotides is a chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis.

Features and Benefits

Industry standard 2′O-TBDMS protective group
Consistent lot-to-lot purity and performance
Compatible with deprotection methods based on methylamine or AMA
Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as an activator; best results are obtained with Activator 42
Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A
Manufactured under a certified ISO 9001 quality system

Other Notes

The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as:

Catalytic agents (ribozymes)
Affinity ligands (aptamers)
Agents to induce gene silencing (RNA interference)

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c