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Merck
CN

G2505

N-Glutaryl-L-phenylalanine p-nitroanilide

protease substrate

Synonym(s):

Glutaryl-L-phenylalanine 4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C20H21N3O6
CAS Number:
5800-34-0
Molecular Weight:
399.40
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
24895096
EC Number:
227-351-4
Beilstein/REAXYS Number:
2919832
MDL number:
MFCD00038362

Key Documents

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Product Name

N-Glutaryl-L-phenylalanine p-nitroanilide, protease substrate

InChI key

LFZGBNATHRHOKZ-KRWDZBQOSA-N

InChI

1S/C20H21N3O6/c24-18(7-4-8-19(25)26)22-17(13-14-5-2-1-3-6-14)20(27)21-15-9-11-16(12-10-15)23(28)29/h1-3,5-6,9-12,17H,4,7-8,13H2,(H,21,27)(H,22,24)(H,25,26)/t17-/m0/s1

SMILES string

OC(=O)CCCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

assay

≥98% (HPLC)

form

powder

solubility

methanol with 1 M NH4OH: 50 mg/mL, clear to slightly hazy

storage temp.

−20°C

Quality Level

100

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Application

N-Glutaryl-L-phenylalanine p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

Biochem/physiol Actions

N-Glutaryl-L-phenylalanine p-nitroanilide is useful in testing α-chymotrypsin activity.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ4284
MKCX5416
MKCX0556
MKCR1562
MKCR0464

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J S Yokhana et al.
Poultry science, 95(3), 550-559 (2015-11-18)
Two experiments were conducted to study effects of dietary insoluble fiber (IF) on digestive enzyme function in layer poultry. In Experiment 1, 8 wk old pullets were fed a control diet (Group C) or a diet (Group IF) supplemented with
Anca Dragulescu-Andrasi et al.
Chemical communications (Cambridge, England), (2)(2), 244-246 (2005-02-23)
Guanidine-based peptide nucleic acid (GPNA) with a d-backbone configuration and alternate spacing binds sequence-specifically to RNA and is readily taken up by both human somatic and embryonic stem (ES) cells.
Takeshi Honda et al.
Chemical communications (Cambridge, England), (40)(40), 5062-5064 (2005-10-13)
A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor
Occurrence and identity of proteolytic bacteria in adult periodontitis.
D Grenier et al.
Journal of periodontal research, 29(5), 365-370 (1994-09-01)
Elsa Abuin et al.
Journal of colloid and interface science, 308(2), 573-576 (2007-01-26)
The rate of N-glutaryl-L-phenylalanine p-nitroanilide hydrolysis catalyzed by alpha-chymotripsin has been measured in aqueous solutions of cetyltrimethylammonium bromide, tetradecyltrimethylammonium bromide, and dodecyltrimethylammonium bromide at concentrations below and above their critical micellar concentrations (CMC). For the three surfactants considered superactivity was
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