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Merck
CN

G4142

Sigma-Aldrich

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

β-D-Galactoside galactohydrolase

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About This Item

CAS Number:
9031-11-2
EC Number:
3.2.1.23 (BRENDA, IUBMB)
EC Number:
232-864-1
MDL number:
MFCD00130623
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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biological source

bovine

Quality Level

200

form

ammonium sulfate suspension

specific activity

1.0-3.0 units/mg protein (modified Warburg-Christian)

foreign activity

α- and β-glucosidase ≤0.2%
α-fucosidase ≤0.2%
α-galactosidase ≤0.2%
α-mannosidase ≤0.2%
β-N-acetylglucosaminidase ≤1.0%

storage temp.

2-8°C

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000375706
0000375706
SLBF3490V
111M7403V
120M7405V

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W Kiess et al.
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
J J Distler et al.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
Elena Piegari et al.
Basic research in cardiology, 108(2), 334-334 (2013-02-16)
The increasing population of cancer survivors faces considerable morbidity and mortality due to late effects of the antineoplastic therapy. Cardiotoxicity is a major limiting factor of therapy with doxorubicin (DOXO), the most effective anthracycline, and is characterized by a dilated
Hongmarn Park et al.
Nucleic acids research, 41(6), 3787-3804 (2013-02-09)
An artificial small RNA (afsRNA) scaffold was designed from an Escherichia coli sRNA, SibC. Using the lacZ reporter system, the gene silencing effects of afsRNAs were examined to explore the sRNA-mediated gene-silencing mechanisms in E. coli. Substitution of the original
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
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