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G4410

Griess reagent (modified)

Synonym(s):

Greiss, nitrite assay

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About This Item

UNSPSC Code:
12352200
EC Number:
215-981-2
NACRES:
NA.32
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form

powder

Quality Level

200

usage

10 g

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000536475
0000534065
0000534029
0000534030
0000520592

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Global Trade Item Number

SKUGTIN
G4410-10G04061835573080