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Giemsa stain

technical grade, used as a blood stain

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Synonym(s):
Azure mixture sicc. Giemsa stain
Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
51811-82-6
Molecular Weight:
291.80
MDL number:
MFCD00081642
PubChem Substance ID:
329799928
NACRES:
NA.47

grade

technical grade

Quality Level

100

form

powder

color

dark greenvery dark

mp

300 °C (lit.)

solubility

ethanol: 1 mg/mL

λmax

645-655 nm

suitability

application for blood stain

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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General description

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa’s staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Application

Giemsa stain has been used for the staining of cells and blood films.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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