G4877
Gonadotropin from pregnant mare serum
≥1,000 IU/mg
Synonym(s):
Equine gonadotropin, PMSG, Pregnant mare serum gonadotropin, eCG
form
powder
specific activity
≥1,000 IU/mg
mol wt
49- 68.5 kDa
solubility
water: 1 mg/mL, clear to slightly hazy, colorless to faintly yellow
storage temp.
−20°C
General description
Gonadotropin hormone is only found in primates and is a member of the glycoprotein hormone family, which also consists of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). This hormone is composed of two non-covalently linked α and β subunits. The α-subunits within a species are identical, whereas the β subunits are different, which confers the differences in physiological specificity.
Application
Gonadotropin from pregnant mare serum has been used-
- for inducing superovulation in female Sprague Dawley rats
- for injection in female golden hamster to eventually obtain zona-free hamster eggs
Biochem/physiol Actions
Gonadotropin interacts with members of the GPCR (G-protein coupled receptor family), and upon activation they induce adenylyl cyclase.
Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.
Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.
Stimulates the growth of ovarian follicles and the formation of the corpus luteum.
Two chain glycoprotein hormone; stimulates the growth of ovarian follicles and the formation of the corpus luteum.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
W F Rall et al.
Journal of reproduction and fertility, 101(3), 681-688 (1994-08-01)
A vitrification solution consisting of 6.5 mol glycerol l-1 and 6% (w/v) BSA in a modified Dulbecco's PBS (designated solution VS3a) was examined for the cryopreservation of 8-12-cell mouse embryos. Solution VS3a vitrified when cooled to -196 degrees C at
P H Chan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(20), 8292-8299 (1998-10-08)
Transient global cerebral ischemia resulting from cardiac arrest is known to cause selective death in vulnerable neurons, including hippocampal CA1 pyramidal neurons. It is postulated that oxygen radicals, superoxide in particular, are involved in cell death processes. To test this
R A Bronson et al.
Biology of reproduction, 43(6), 1019-1025 (1990-12-01)
The Arg-Gly-Asp (RGD) sequence is known to play a role in many recognition systems involved in cell-to-cell and cell-to-matrix adhesion. In our experiments we demonstrated that an RGD-dependent recognition is involved in sperm-oolemmal adhesion and egg penetration. Following coincubation of
T Braun et al.
The EMBO journal, 10(7), 1885-1890 (1991-07-01)
Recombinant expression of truncated receptors for luteinizing hormone/chorionic gonadotropin (LH/CG) revealed that the amino-terminal leucine-rich repeats 1-8 of the extracellular receptor domain bind human chorionic gonadotropin (hCG) with an affinity (Kd = 0.72 +/- 0.2 nM) similar to that of
Mario Plaas et al.
Scientific reports, 7(1), 10220-10220 (2017-09-02)
Wolfram syndrome (WS) is a rare autosomal-recessive disorder that is caused by mutations in the WFS1 gene and is characterized by juvenile-onset diabetes, optic atrophy, hearing loss and a number of other complications. Here, we describe the creation and phenotype
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service