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G5537

Glyceraldehyde-3-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder, 70-140 units/mg protein

Synonym(s):

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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About This Item

CAS Number:
9001-50-7
UNSPSC Code:
12352204
EC Number:
232-609-4
MDL number:
MFCD00131203
EC Number:
1.2.1.12 (BRENDA, IUBMB)
Specific activity:
70-140 units/mg protein
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form

lyophilized powder

Quality Segment

200

specific activity

70-140 units/mg protein

composition

Protein, 30-70% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%

storage temp.

−20°C

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.

Physical form

Lyophilized, sulfate-free powder stabilized with trehalose, citrate, and DTT. Useful for systems requiring low sulfate.

Other Notes

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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