G5671
Anti-Galectin-8 antibody, Mouse monoclonal
~1.0 mg/mL, clone VA-11.25, purified from hybridoma cell culture
Synonym(s):
ANTIGal-8, ANTILGALS8, ANTILectin, galectoside-binding, soluble 8, ANTIPCTA1, ANTIProstate carcinoma tumor antigen 1
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
VA-11.25, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
7
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
VA-11.25, monoclonal
form
buffered aqueous solution
mol wt
antigen ~34 kDa
species reactivity
mouse, rat, human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1-2 μg/mL using rat galectin 8 recombinant protein
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LGALS8(3964)
mouse ... Lgals8(56048)
rat ... Lgals8(116641)
General description
Galectins specifically bind to β-galactosides. 15 mammalian galectins have been identified so far. Galectin 8 contains two CRDs connected by a short linker peptide.
Monoclonal Anti-Galectin 8 (mouse IgM isotype) is derived from the hybridoma VA-11.25 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant rat galectin 8.Galectins belong to a family of carbohydrate-binding proteins. Within this family of proteins, galectin 8 is unique, as it exists in many forms encoded by the same gene.
Immunogen
recombinant rat galectin 8.
Application
Monoclonal Anti-galectin-8 antibody produced in mouse has been used for:
- immunoblotting
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Galectins belong to a family of carbohydrate-binding proteins. These proteins share similarities in the carbohydrate recognition domain (CRD) and in their specificity for N-Acetyllactosamine-enriched glycoconjugates. The binding of galectins to saccharides modulates cell proliferation, cell death and cell migration, which are involved in cancer initiation and progression. Galectin 8 is a secreted protein that requires both of its CRDs to be active to modulate cell adhesion. It forms a complex with integrins and triggers integrin-mediated signaling cascades. On the contrary, excessive amounts of this protein negatively regulate cell adhesion by interacting with integrins. These signals assist in identifying regions available for cell adhesion and migration. It may be involved in neoplastic transformation.
The antibody has low cross-reactivity with galectin 3 and galectin 9.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
低风险生物材料
常规特殊物品
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
G A Rabinovich
Cell death and differentiation, 6(8), 711-721 (1999-09-01)
Galectins constitute a family of evolutionarily conserved animal lectins, which are defined by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain. During the past decade, attempts to dissect the functional role for galectins in vivo
Human galectin-8 isoforms and cancer
Bidon WN and Le Pennec JP
Glycoconjugate Journal, 19(7-9), 557-563 (2002)
Y Levy et al.
The Journal of biological chemistry, 276(33), 31285-31295 (2001-05-24)
The interaction of cells with the extracellular matrix regulates cell adhesion and motility. Here we demonstrate that different cell types adhere and spread when cultured in serum-free medium on immobilized galectin-8, a mammalian beta-galactoside-binding protein. At maximal doses, galectin-8 is
Hitomi Yamamoto et al.
Journal of biochemistry, 143(3), 311-324 (2007-11-21)
We previously showed that tandem-repeat type galectin-8, which has two covalently linked carbohydrate recognition domains (CRDs), induces neutrophil-adhesion through binding to integrin alphaM. Here, we analysed the function of galectin-8 in Jurkat T-cells. Galectin-8, as well as tandem-repeat galectin-9, and
Laurent Ingrassia et al.
Current medicinal chemistry, 13(29), 3513-3527 (2006-12-16)
Galectins form a family of carbohydrate-binding proteins defined by their affinity for beta-galactosides containing glycoconjugates. The carbohydrate recognition domain (CRD) is responsible for the specificity of galectins for saccharides. This binding may result in modulated cell proliferation, cell death and
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G5671-200UL | 04061837873348 |
| G5671-100UL | 04061838031198 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service