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G5768

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Synonym(s):

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

NACRES:
NA.72
UNSPSC Code:
12352207
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form

fine powder

Quality Segment

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer
  • as a media to maintain potato suspension and callus cultures
  • as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Preparation Note

Formulated to contain 3.1 grams of powder per liter of medium.
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation


Pictograms

Flame over circle

Signal Word

Warning

Hazard Codes

Hazard Classifications

Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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