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Merck
CN

G6378

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Synonym(s):

G-6-P-DH, Zwischenferment

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
EC Number:
Specific activity:
200-400 units/mg protein (modified Warburg-Christian)
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type

Type XV

Quality Segment

form

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

mol wt

128 kDa

purified by

crystallization

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight

Preparation Note

Prepared from G7877

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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