G6378
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym(s):
G-6-P-DH, Zwischenferment
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
200-400 units/mg protein (modified Warburg-Christian)
type
Type XV
Quality Level
form
lyophilized powder
specific activity
200-400 units/mg protein (modified Warburg-Christian)
mol wt
128 kDa
purified by
crystallization
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
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General description
Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).
Application
Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight
Preparation Note
Prepared from G7877
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
María J Rodríguez-López et al.
Insects, 11(9) (2020-09-13)
A combination of biological control and host plant resistance would be desirable for optimally controlling the greenhouse whitefly, Trialeurodes vaporariorum in tomato crops. Whitefly settlement preference, oviposition, and survivorship were evaluated on ABL 10-4 and 'Moneymaker', two nearly-isogenic tomato lines
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
Elisa Principi et al.
Cancers, 14(7) (2022-04-13)
RNF5, an endoplasmic reticulum (ER) E3 ubiquitin ligase, participates to the ER-associated protein degradation guaranteeing the protein homeostasis. Depending on tumor model tested, RNF5 exerts pro- or anti-tumor activity. The aim of this study was to elucidate the controversial role
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
Protocols
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G6378-100UN | 04061833639054 |
| G6378-2KU | 04061833639085 |
| G6378-500UN | 04061833639092 |
| G6378-250UN | 04061833639078 |
| G6378-5KU | 04061833639108 |
| G6378-1KU | 04061833639061 |
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