G7765
Gelatin from cold water fish skin
40-50% in H2O
Synonym(s):
Gelatine, Teleostean gelatin
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About This Item
biological source
fish skin
Quality Segment
form
liquid
mol wt
~60 kDa
contains
0.15% propyl p-hydroxybenzoate as preservative, 0.2% methyl p-hydroxybenzoate as preservative
concentration
40-50% in H2O
technique(s)
ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable
storage temp.
2-8°C
General description
Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.
Application
Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.
Used as a blocking agent in immunochemistry.
Biochem/physiol Actions
Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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