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G8529

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Name: Glucose-6-phosphate Dehydrogenase from <I>Leuconostoc mesenteroides</I>
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:

9001-40-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-602-6

MDL number:

MFCD00081656

EC Number:

1.1.1.49 (BRENDA, IUBMB)

Specific activity:

≥550 units/mg protein (biuret)

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Level

300

form

lyophilized powder

specific activity

≥550 units/mg protein (biuret)

composition

Protein, 10-40% biuret

application(s)

agriculture

foreign activity

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

storage temp.

2-8°C

Description

Application

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
to study the starch time course using Nicotiana tabacum leaves
to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000502013

0000453963

0000406497

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Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

Global Trade Item Number

SKU	GTIN
G8529-2KU	04061833644096
G8529-200UN	04061832730332
G8529-1KU	04061833644089
G8529-10KU	04061832730318