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G9654

Gentamicin-Glutamine

Name: Gentamicin-Glutamine
Brand: SIGMA

solution, suitable for cell culture, BioReagent

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.76

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Properties

Product Name

Gentamicin-Glutamine solution, with 200 mM L-glutamine and 5 mg gentamicin per mL in tissue culture grade water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Quality Level

300

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

potency

5-6 mg per mL

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycoplasma

mode of action

protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

Description

General description

Chemical structure: aminoglycoside

Application

Gentamicn-Glutamine solution is used for selection of transformed cells that contain the aacA.aphD gene, and is recommended for use in cell culture applications at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit. Glutamine functions as an essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeletons.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.

Other Notes

This solution is formulated to contain 200 mM L-glutamine and 5 mg/mL gentamicin in .9% sodium chloride

Disclaimer

This product is stable in solution at 37°C for 5 days. The recommended storage temperature is -20°C.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

0000428425

0000221840

0000133202

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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

L-谷氨酰胺在细胞培养中的应用

L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用

Role of long non-coding RNA in DEET- and fipronil-mediated alteration of transcripts associated with Phase I and Phase II xenobiotic metabolism in human primary hepatocytes.

Roger D Lawrie et al.

Pesticide biochemistry and physiology, 167, 104607-104607 (2020-06-13)

Human exposure to environmental chemicals both individually and in combination occurs frequently world-wide most often with unknown consequences. Use of molecular approaches to aide in the assessment of risk involved in chemical exposure is a growing field in toxicology. In

Global Trade Item Number

SKU	GTIN
G9654-10X5ML	04061833650325