G9768
Guanosine 5′-triphosphate–Agarose
aqueous glycerol suspension
Synonym(s):
5′-GTP agarose
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About This Item
Biological source:
bacterial (CORYNEBACTERIUM SP), enzyme from Porcine brain, plant (Sea weed), yeast
Form:
aqueous glycerol suspension
Storage temp.:
−20°C
Quality Segment
biological source
bacterial (CORYNEBACTERIUM SP), enzyme from Porcine brain, plant (Sea weed), yeast
form
aqueous glycerol suspension
extent of labeling
1-5 μmol per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
ribose hydroxyls
matrix spacer
11 atoms (adipic acid dihydrazide)
storage temp.
−20°C
SMILES string
[X]O[C@H]1[C@@H](O[C@@H]([C@H]1O[X])CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O)[n]2c3nc(nc(c3nc2)O)N
Application
Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis
Physical form
Guanosine 5′-triphosphate–Agarose has been used:
- in chromatographic column for the purification of a translocation stimulating factor
- in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
- in GTP-Binding assay
- for the depletion of GTP binding proteins in cytosol of rat liver
Suspension in 50% glycerol containing 0.25 M NaCl
Storage Class
10 - Combustible liquids
wgk
WGK 3
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