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GAGO20

Sigma-Aldrich

Glucose (GO) Assay Kit

sufficient for 20 assays

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NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

100

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Related Categories

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Suitability

Suitable for the quantitative, enzymatic determination of glucosein food and other materials.

Kit Components Only

Product No.
Description
  • o-dianisidine reagent 1 mL/vial
  • glucose oxidase/peroxidase reagent 1 capsule
  • glucose standard .5 mL

Signal Word

Danger

Hazard Statements

H302 - H314 - H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Information

危险化学品
常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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  1. Can Product GAGO20, Glucose (GO) Assay Kit, be tested on blood or plasma glucose?

    For analysis of such samples, this kit should be useful on samples that have been deproteinized. 

  2. Can Product GAGO20, Glucose (GO) Assay Kit, be used in a 96-well plate format?

    Sigma Aldrich has not performed this in a 96-well plate format.  However if a method has have not tried this. If you have developed a method in a 96-well format, Sigma Aldrich would be happy to know about this.

  3. Can frozen samples be tested with Product GAGO20, Glucose (GO) Assay Kit?

    One publication has successfully reported use of the GAGO20 on samples frozen at -20 °C.

  4. Can Product GAGO20, Glucose (GO) Assay Kit, be used on culture media samples?

    Sigma Aldrich has not tested this product on culture media samples, but several publications in the literature report successful use of the kit with culture media samples.

  5. What is the difference between the GAGO20 and GAHK20 Glucose (GO) Assay Kits?

    The kits use different enzymes. GAGO20 uses glucose oxidase and peroxidase, while GAHK20 uses hexokinase.  Furthermore, the final reaction products are monitored at different wavelengths, 540 nm (GAGO20) and 340 nm (GAHK20).

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. How would I decolorize my sample for use in the glucose assay using Product GAGO20, Glucose (GO) Assay Kit?

    First, you will need to determine if the color affects the result by reading it as a blank in the assay. The classical method for decolorization of carbohydrate solutions is treatment with charcoal. A small amount of powdered charcoal such as Product C3345 is added to the sample, stirred a few moments, then clarified by filtration or centrifugation. If the color persists, another portion of charcoal is added and the process repeated until there is no more change in color with an addition of charcoal.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Bergmeyer, H.U. and Bernt, E.,
Methods of Enzymatic Analysis, 1205-1212 (1974)
S Talukder et al.
Journal of food science and technology, 57(2), 537-548 (2020-03-03)
Natural plant pigment, anthocyanins have the capability to change its color with the change of its structure influenced by changing pH. This feature of anthocyanin has been harnessed to design a meat products quality indicator. In the present experiment anthocyanin
M Isabel Muro-Pastor et al.
Plant physiology, 184(4), 1792-1810 (2020-09-10)
Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown-as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but
Nelson H Knudsen et al.
Science (New York, N.Y.), 368(6490) (2020-05-02)
Repeated bouts of exercise condition muscle mitochondria to meet increased energy demand-an adaptive response associated with improved metabolic fitness. We found that the type 2 cytokine interleukin-13 (IL-13) is induced in exercising muscle, where it orchestrates metabolic reprogramming that preserves
David Ohayon et al.
Nature materials, 19(4), 456-463 (2019-12-18)
A promising class of materials for applications that rely on electron transfer for signal generation are the n-type semiconducting polymers. Here we demonstrate the integration of an n-type conjugated polymer with a redox enzyme for the autonomous detection of glucose
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