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Merck
CN

GE17-0090-10

Sephadex® LH-20

Cytiva 17-0090-10, pack of 25 g

Synonym(s):

Sephadex gel

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151817

Key Documents

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Technical Service
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Product Name

Sephadex® LH-20, Cytiva 17-0090-10, pack of 25 g

form

resin

crosslinking

cross-linked

mol wt

protein Mr 4-5 kDa (fractionation range)
Mr 5 kDa (proteins and organics exclusion limit)

feature

autoclavable 20 min at 121°C

packaging

pack of 25 g

manufacturer/tradename

Cytiva 17-0090-10

parameter

60 cm/hr flow rate (recommended)
700 cm/hr max. flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

hydroxypropylated, cross-linked dextran

matrix active group

dextran

particle size

103 μm (in methanol)
70 μm (dry)

cleaning in place

2-13

working range

2-13

separation technique

size exclusion (SEC)

Related Categories

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed Dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and Low molecular weight peptides, in organic solvents.

Features and Benefits

  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex® LH-20 is made from hydroxypropylated Dextran beads that have been crosslinked to yield a polysaccharide network.
  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Excellent batch-to-batch reproducibility
  • Sephadex® LH-20 is made from hydroxypropylated Dextran beads that have been crosslinked to yield a polysaccharide network.

General description

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 25 °C ()

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

13 - Non Combustible Solids

Regulatory Information

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Protocols

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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