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Merck
CN

GE17-0180-01

DEAE-Sephadex® A-50

Cytiva 17-0180-01, pack of 100 g

Synonym(s):

IEX resin, chromatography resin, diethylaminoethyl resin, gel, ion exchange resin, matrix, weak anion resin

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151817

Key Documents

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description

Ion Exchanger Type (value)

form

resin

feature

autoclavable In 0.1 M sodium chloride at 121°C for 30 min.

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0180-01

storage condition

dry media

parameter

≥60 cm/hr flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

matrix active group

diethylaminoethyl (DEAE)

particle size

40-120 μm (dry)

cleaning in place

2-12

working range

2-12

capacity

3-4 mmol/g binding capacity (dry resin)

separation technique

weak anion exchange

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General description

DEAE Sephadex® A-50 is a weak anion exchanger based on the well documented and well proven Sephadex® base matrix and widely used especially in plasma fractionation applications.
thyroglobulin (Mr 669 000)- 2 mg/ml drained medium), HSA (Mr 68 000)- 110 mg/ml drained medium), α-lactalbumin (Mr 14 300)- 50 mg/ml drained medium)

Application

Sephadex® ion exchangers are produced by introducing functional groups onto the cross-linked Dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.


Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.

Features and Benefits

  • Weak anion exchanger suitable batch techniques.
  • High binding capacities.
  • Well-proven and widely used especially in approved plasma fractionation processes.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Storage Class

13 - Non Combustible Solids

Regulatory Information

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Protocols

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

使用Sephadex®进行分离

本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。

Related Content

Separation Options with Sephadex®

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

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