description
Ion Exchanger Type (value)
form
resin
feature
autoclavable In 0.1 M sodium chloride at 121°C for 30 min.
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0180-01
storage condition
dry media
parameter
≥60 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
matrix active group
diethylaminoethyl (DEAE)
particle size
40-120 μm (dry)
cleaning in place
2-12
working range
2-12
capacity
3-4 mmol/g binding capacity (dry resin)
separation technique
weak anion exchange
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General description
DEAE Sephadex® A-50 is a weak anion exchanger based on the well documented and well proven Sephadex® base matrix and widely used especially in plasma fractionation applications.
thyroglobulin (Mr 669 000)- 2 mg/ml drained medium), HSA (Mr 68 000)- 110 mg/ml drained medium), α-lactalbumin (Mr 14 300)- 50 mg/ml drained medium)
Application
Sephadex® ion exchangers are produced by introducing functional groups onto the cross-linked Dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.
Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.
Features and Benefits
- Weak anion exchanger suitable batch techniques.
- High binding capacities.
- Well-proven and widely used especially in approved plasma fractionation processes.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
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实验方案
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。
相关内容
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
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