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About This Item
NACRES:
NA.56
UNSPSC Code:
23151816
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-0402-01
storage condition
(20% Ethanol)
parameter
0.5 Mpa max. pressure, 1 mL/min flow rate, 4 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked agarose, spherical
matrix active group
Protein A
avg. part. size
34 μm
cleaning
2-10
working range
3-9
capacity
~20 mg binding capacity (human IgG / mL resin)
separation technique
affinity
storage temp.
2-8°C
General description
HiTrap® Protein A HP is a prepacked Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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