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Merck
CN

GE17-0756-04

Glutathione Sepharose™ 4B

Cytiva 17-0756-04, pack of 300 mL

Synonym(s):

Glutathione Column

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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Technical Service
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packaging

pack of 300 mL

manufacturer/tradename

Cytiva 17-0756-04

storage condition

(20% ethanol)

parameter

<100 cm/hr flow rate (sample loading)
100-300 cm/hr flow rate (wash and elution)

technique(s)

liquid chromatography (LC): suitable

matrix

4% agarose

matrix active group

Glutathione

average diameter

90 μm

cleaning in place

4-13

working range

4-13

capacity

>25 mg binding capacity (recombinant GST / ml)

separation technique

affinity

shipped in

ambient

storage temp.

2-30°C

Related Categories

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

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