form
resin
crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0906-01
storage condition
(100% Isopropanol)
parameter
150-250 cm/hr flow rate (at 0.1 MPa)
technique(s)
liquid chromatography (LC): suitable
matrix
4% cross-linked agarose
matrix active group
N-hydroxysuccinimide (NHS) activated
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-13(ligand dependent)
operating pH
2-9
separation technique
affinity
storage temp.
2-8°C
General description
NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.
This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.
NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.
This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.
NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation gives a high degree of substitution of the selected ligand
- NHS coupling method results in chemically stable ligand attachment
- BioProcess medium supported for industrial applications
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Danger
hcodes
存储类别
3 - Flammable liquids