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Merck
CN

GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL

别名:

NHS-Activated Sepharose FF, pre-activated agarose matrix

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NACRES:
NA.56
UNSPSC Code:
41106500

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产品名称

NHS Act Sepharose™ 4 Fast Flow, Cytiva 17-0906-01, pack of 25 mL

form

resin

crosslinking

cross-linked

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0906-01

storage condition

(100% Isopropanol)

parameter

150-250 cm/hr flow rate (at 0.1 MPa)

technique(s)

liquid chromatography (LC): suitable

matrix

4% cross-linked agarose

matrix active group

N-hydroxysuccinimide (NHS) activated

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-13(ligand dependent)

operating pH

2-9

separation technique

affinity

storage temp.

2-8°C

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

General description

NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

Sepharose is a trademark of Cytiva

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Exclamation mark
GHS07

signalword

Danger

存储类别

3 - Flammable liquids

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抗体亲和色谱的脱盐和缓冲液置换

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实验方案

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

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