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Merck
CN

GE17-1194-01

Sigma-Aldrich

HiPrep™ 26/60 Sephacryl® S-100 HR

Cytiva 17-1194-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

Synonym(s):

SEC column, chromatography tube, cytiva 17-1194-01, prepacked column, size-exclusion column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Key Documents

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material

polypropylene

mol wt

10000-100000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1194-01

parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa pressure (22 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

Related Categories

General description

HiPrep™ 26/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.

Application

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Protocols

Sephacryl® Separation

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

使用Sephacryl®进行分离

本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。

Related Content

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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