material
polypropylene
mol wt
10000-100000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-1194-01
parameter
<13 mL sample volume, <2.6 mL/min flow rate, 0.15 Mpa pressure (22 psi)
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric
matrix active group
dextran-acrylamide phase
avg. part. size
50 μm
cleaning
2-13
working range
3-11
separation technique
size exclusion (SEC)
General description
HiPrep™ 26/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.
Application
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume
Features and Benefits
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
- Gel filtration columns for low to medium pressure AKTA systems.
- Well-established in industrial production.
- The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
此项目有
实验方案
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
This page shows how to refold proteins using gel filtration and products from Cytiva.
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离,该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
相关内容
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE17-1194-01 | 04061838214812 |
