Skip to Content
Merck
CN

GE17-1195-01

HiPrep 26/60 Sephacryl® S-200 HR

Cytiva 17-1195-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
SB.52
UNSPSC Code:
41115700
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


material

polypropylene (PP)

mol wt

5000-250000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1195-01

parameter

<13 mL sample volume, <2.6 mL/min flow rate, 0.15 Mpa (22 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

General description

HiPrep 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.

Application

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

Preparation Note

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva


pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library