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HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL


shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 5 × 5 mL


Cytiva 17-1408-01

storage condition

dry media


<15 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm


cross-linked dextran

particle size

>20 μm (dry)



working range



suitable for bioprocess medium

Related Categories

General description

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.


Desalting and buffer exchange with Sephadex® G-25.

Features and Benefits

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

Storage and Stability

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit

Legal Information

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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