form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 5 kDa (exclusion limit)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1408-01
storage condition
(20% ethanol)
parameter
0.1-1.5 mL sample volume, 1-10 mL/min flow rate, 15 mL/min max. flow rate, 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
cross-linked dextran
matrix active group
dextran
particle size
20-50 μm (dry)
cleaning
2-13
working range
2-13
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
Application
Desalting and buffer exchange with Sephadex® G-25.
Features and Benefits
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids