form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 5 kDa (exclusion limit)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1408-01
storage condition
(20% ethanol)
parameter
0.1-1.5 mL sample volume, 1-10 mL/min flow rate, 15 mL/min max. flow rate, 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
cross-linked dextran
matrix active group
dextran
particle size
20-50 μm (dry)
cleaning
2-13
working range
2-13
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
Application
Features and Benefits
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
Preparation Note
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
实验方案
This page shows how to prepare samples for purification with affinity chromatography.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE17-1408-01 | 04061837621437 |