表单
prepacked column
交联
cross-linked
分子量
protein Mr 5 kDa (exclusion limit)
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-1408-01
储存条件
(20% ethanol)
参数
0.1-1.5 mL sample volume
1-10 mL/min flow rate
15 mL/min max. flow rate
5 bar max. pressure
技术
liquid chromatography (LC): suitable
柱床体积
5 mL
色谱柱内径 × 长度
16 mm × 25 mm
基质
cross-linked dextran
基质活性基团
dextran
粒径
20-50 μm (dry)
cleaning
2-13
工作范围
2-13
分离技术
size exclusion (SEC)
运输
ambient
储存温度
2-30°C
一般描述
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
应用
特点和优势
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
制备说明
分析说明
其他说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
商品
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This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
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实验方案
This page shows how to prepare samples for purification with affinity chromatography.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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