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Merck
CN

GE17-1440-02

Ficoll® Paque Plus

liquid, sterile (endotoxins < 0.12 EU/mL), density: 1.077 g/mL, pack of 6 × 100 mL

Synonym(s):

17-1440-02, Ficoll density gradient medium, cell culture media, cell purification media, cell storage media, density gradient media, lymphocyte separation medium, mononuclear cell isolation

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About This Item

UNSPSC Code:
41105330
NACRES:
NA.25

Key Documents

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sterility

sterile (endotoxins < 0.12 EU/mL)

form

liquid

shelf life

3 yr

packaging

pack of 6 × 100 mL

manufacturer/tradename

Cytiva 17-1440-02

storage condition

protect from light ()

dilution

(purification of mononuclear cells from human peripheral blood)

color

Colorless to slightly yellow

pH range

5.5-7.5

density

1.077 g/mL

Related Categories

General description

Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies.

Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.

Application

For in vitro isolation of lymphocytes from human peripheral blood.

Features and Benefits

  • Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood.
  • Maintains viability and representative distribution of B and T lymphocytes.
  • Low levels of endotoxin (< 0.12 EU/mL).

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H225

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Regulatory Information

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Articles

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocols

Ficoll® 400 for Gradient Centrifugation

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用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

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