ligand
matrix surface
material
polypropylene column
feature
autoclavable 20 min at 121°C
packaging
pkg of 1 column
parameter
flow rate, 0.5 ml/min - 2.5 ml/min flow rate, 580 psi
technique(s)
HPLC: suitable
bed size
4.6 mm × 100 mm
bed volume
1.7 mL
column I.D.
4.6 mm
matrix
polymeric, polystyrene/divinylbenzene
matrix active group
polystyrene-divinylbenzene phase
avg. part. size
15 μm
cleaning
1-14
working range
1-12
capacity
~10 mg binding capacity
suitability
suitable for bioprocess medium
separation technique
reversed phase
General description
SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h
Application
SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.
Features and Benefits
- Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
- Excellent scalability, from RESOURCE™ to FineLINE columns.
- using ÄKTA® design and other high-performance liquid chromatography systems.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids