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Merck
CN

GE17-5068-01

SOURCE™ 15RPC ST 4.6/100

pkg of 1 column

别名:

chromatography cartridge, cytiva 17-5068-01, prepacked column, RPC resin, reverse phase chromatography column

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NACRES:
SB.52
UNSPSC Code:
41115700

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产品名称

SOURCE™ 15RPC ST 4.6/100, pkg of 1 column

ligand

matrix surface

material

polypropylene column

feature

autoclavable 20 min at 121°C

packaging

pkg of 1 column

parameter

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

technique(s)

HPLC: suitable

bed size

4.6 mm × 100 mm

bed volume

1.7 mL

column I.D.

4.6 mm

matrix

polymeric
polystyrene/divinylbenzene

matrix active group

polystyrene-divinylbenzene phase

avg. part. size

15 μm

cleaning

1-14

working range

1-12

capacity

~10 mg binding capacity

suitability

suitable for bioprocess medium

separation technique

reversed phase

相关类别

Legal Information

ÄKTA is a registered trademark of Cytiva
RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.

Features and Benefits

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE™ to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

General description

SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

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商品

RPC Purification Options and Scale up with SOURCE™ Media

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

Troubleshooting Reversed Phase Chromatography

“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

实验方案

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

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