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Merck
CN

GE17-5112-01

HiTrap® Streptavidin High Performance

Cytiva 17-5112-01, pack of 5 × 1 mL

Synonym(s):

HiTrap Streptavidin, Streptavidin High Performance

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816

Key Documents

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working range

4-9

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5112-01

storage condition

(20% Ethanol), (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8)

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10.5

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

Related Categories

General description

HiTrap® Streptavidin HP is a column prepacked with Streptavidin Sepharose™ High Performance for purification of biotinylated proteins and biomolecules.

Application

The prepacked Streptavidin Sepharose™ High Performance medium ensures reproducibility, high performance, and excellent physical and chemical stability. Streptavidin Sepharose™ High Performance is also available in lab packs, using the same purified streptavidin isolated from Streptomyces avidinii bound to Sepharose™ High Performance.

Features and Benefits

  • Convenient, fast, reliable purification of biotinylated compounds.
  • The strong interactions of biotin and streptavidin, as well as the somewhat weaker interactions of 2-iminobiotin and streptavidin are utilized.
  • The column can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

pictograms

Flame
GHS02

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Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocols

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance

How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from Cytiva.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

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