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Merck
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GE17-5130-02

GSTrap™ Fast Flow

Cytiva 17-5130-02, pack of 2 × 1 mL

Synonym(s):

GST-tagged purification columns, affinity column, chromatography cartridge, glutathione Sepharose 4 FF, prepacked column

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About This Item

NACRES:
NA.25
UNSPSC Code:
23151817

Key Documents

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Technical Service
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separation technique

affinity

form

prepacked column

crosslinking

cross-linked

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-5130-02

parameter

0.3-0.5 mL/min flow rate (sample loading), 0.5 Mpa max. pressure, 1.5 mL/min flow rate (wash and elution)

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

matrix

highly cross-linked 4% agarose

matrix active group

glutathione

particle size

~90 μm

cleaning

3-12

operating pH

3-12

capacity

>10 mg/mL binding capacity

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General description

GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

Features and Benefits

  • Fast and simple one-step purification of GST-tagged Proteins.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose™ 4 Fast FLow provides good fLow properties.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

See All

Protocols

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service