form
prepacked column
crosslinking
cross-linked
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 17-5130-02
parameter
0.3-0.5 mL/min flow rate (sample loading), 0.5 Mpa max. pressure, 1.5 mL/min flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked 4% agarose
matrix active group
glutathione
particle size
~90 μm
cleaning
3-12
operating pH
3-12
capacity
>10 mg/mL binding capacity
separation technique
affinity
General description
GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Application
GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.
Features and Benefits
- Fast and simple one-step purification of GST-tagged Proteins.
- Mild elution conditions preserving Protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast FLow provides good fLow properties.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
Still not finding the right product?
Explore all of our products under GSTrap™ Fast Flow
signalword
Warning
hcodes
存储类别
3 - Flammable liquids