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About This Item
separation technique
affinity
storage temp.
2-30°C
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5131-02
storage condition
(20% ethanol)
parameter
0.5 Mpa max. pressure, 1.5-2.5 mL/min flow rate (sample loading), 7 mL/min flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
highly cross-linked 4% agarose
matrix active group
glutathione
particle size
~90 μm
cleaning
3-12
operating pH
3-12
capacity
>10 mg/mL binding capacity
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General description
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
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Features and Benefits
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
Preparation Note
Analysis Note
Other Notes
Legal Information
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Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Protocols
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
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