ligand
glutathione and 10-carbon linker arm
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-5131-02
参数
flow rate
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
highly cross-linked 4% agarose
平均直径
90 μm
cleaning
3-12
工作范围
3-12
容量
>10 mg/mL binding capacity
相关类别
一般描述
GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
特点和优势
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持