GE17-5132-03
Glutathione Sepharose™ 4 Fast Flow
Cytiva 17-5132-03, pack of 500 mL
Synonym(s):
Glutathione Column, Sepharose 4 Fast Flow
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About This Item
form
resin
crosslinking
cross-linked
packaging
pack of 500 mL
manufacturer/tradename
Cytiva 17-5132-03
storage condition
(20% ethanol)
parameter
100 cm/hr max. flow rate (sample loading)
100-300 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 4% agarose
matrix active group
Glutathione
particle size
~90 μm
cleaning
3-12
operating pH
3-12
capacity
>10 mg/mL binding capacity
separation technique
affinity
storage temp.
2-30°C
Related Categories
General description
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
Features and Benefits
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
Preparation Note
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
Protocols
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
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