表单
resin
交联
cross-linked
包装
pack of 500 mL
制造商/商品名称
Cytiva 17-5132-03
储存条件
(20% ethanol)
参数
100 cm/hr max. flow rate (sample loading)
100-300 cm/hr flow rate
技术
liquid chromatography (LC): suitable
基质
highly cross-linked 4% agarose
基质活性基团
Glutathione
粒径
~90 μm
cleaning
3-12
工作pH值
3-12
容量
>10 mg/mL binding capacity
分离技术
affinity
储存温度
2-30°C
一般描述
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
特点和优势
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
制备说明
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
实验方案
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持