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Merck
CN

GE17-5194-01

HiTrap® Phenyl Fast Flow (LS)

Cytiva 17-5194-01, pack of 5 × 5 mL

Synonym(s):

HIC purification column, aromatic HIC matrix, chromatography cartridge, hydrophobic interaction chromatography resin, phenyl Sepharose FF, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816

Key Documents

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Technical Service
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ligand

phenyl

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5194-01

storage condition

(20% Ethanol)

parameter

42 psi

technique(s)

liquid chromatography (LC): suitable

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

matrix active group

phenyl functional group

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

separation technique

hydrophobic interaction (HIC)

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General description

HiTrap® Phenyl FF (Low Sub) are prepacked Phenyl Sepharose™ 6 Fast FLow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

Features and Benefits

  • Provide convenient and fast small-scale Protein purifications.
  • Phenyl Sepharose™ 6 Fast FLow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring Low to medium hydrophobicity. Two levels of substitution on Phenyl Sepharose™ 6 Fast FLow help find optimal selectivity and binding capacity.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
  • High fLow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
HiPrep is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Protocols

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service