ligand
phenyl
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5194-01
storage condition
(20% Ethanol)
parameter
42 psi
technique(s)
liquid chromatography (LC): suitable
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
matrix active group
phenyl functional group
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium
separation technique
hydrophobic interaction (HIC)
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General description
HiTrap® Phenyl FF (Low Sub) are prepacked Phenyl Sepharose™ 6 Fast FLow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Features and Benefits
- Provide convenient and fast small-scale Protein purifications.
- Phenyl Sepharose™ 6 Fast FLow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring Low to medium hydrophobicity. Two levels of substitution on Phenyl Sepharose™ 6 Fast FLow help find optimal selectivity and binding capacity.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
- High fLow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
HiPrep is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
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商品
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
实验方案
This page discusses various aspects of sample preparation for chromatographic purification.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
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