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About This Item
Product Name
HisTrap™ Fast Flow, Cytiva 17-5255-01, pack of 5 × 5 mL
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5255-01
storage condition
(20% Ethanol)
parameter
20 mL/min max. flow rate
5 bar max. pressure
5 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed I.D. × L
16 mm × 25 mm
bed volume
5 mL
matrix
6% cross-linked agarose
matrix active group
Ni2+ ions
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein / mL)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
Related Categories
Analysis Note
Features and Benefits
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
General description
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
How to optimize purification of histidine-tagged proteins using Cytiva products.
Protocols
This page shows how to purify protein A-tagged proteins using Cytiva products.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
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