HisTrap^™ Fast Flow

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Storage Temp.: 4°C to 30°C

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva