GE17-5255-01

HisTrap^™ Fast Flow

Name: HisTrap<SUP>™</SUP> Fast Flow
Brand: SIGMA

Cytiva 17-5255-01, pack of 5 × 5 mL

别名:

HisTrap FF, His-tagged protein purification column, ready-to-use column

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NACRES:

NA.56

UNSPSC Code:

23151816

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产品名称

HisTrap^™ Fast Flow, Cytiva 17-5255-01, pack of 5 × 5 mL

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5255-01

storage condition

(20% Ethanol)

parameter

20 mL/min max. flow rate
5 bar max. pressure
5 mL/min flow rate (recommended)

technique(s)

liquid chromatography (LC): suitable

bed I.D. × L

16 mm × 25 mm

bed volume

5 mL

matrix

6% cross-linked agarose

matrix active group

Ni²⁺ions

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni²⁺-stripped medium.)

working range

3-12(Ni²⁺-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein / mL)

separation technique

affinity

shipped in

ambient

storage temp.

2-30°C

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

High binding capacity, approx. 40 mg/mL medium.
Negligible leakage of Ni²⁺.
Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

General description

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Preparation Note

Storage Temp.: 4°C to 30°C

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)