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About This Item
Product Name
Ni Sepharose™ High Performance, Cytiva 17-5268-01, pack of 25 mL
crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5268-01
storage condition
(20% ethanol)
parameter
150 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 6% agarose
matrix active group
Nickel
particle size
~34 μm
cleaning in place
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni²⁺ stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein / ml medium)
separation technique
affinity
storage temp.
2-30°C
Related Categories
Preparation Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Analysis Note
Features and Benefits
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
General description
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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