crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5268-01
storage condition
(20% ethanol)
parameter
150 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 6% agarose
matrix active group
Nickel
particle size
~34 μm
cleaning in place
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni²⁺ stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein / ml medium)
separation technique
affinity
storage temp.
2-30°C
General description
Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Features and Benefits
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
此项目有
商品
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE17-5268-01 | 04061837622083 |