包装
pack of 25 mL
制造商/商品名称
Cytiva 17-5268-01
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning in place
2-14(Ni2+-stripped medium.)
工作范围
3-12(Ni²⁺-stripped medium.)
容量
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
一般描述
Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
特点和优势
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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