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About This Item
form
resin
crosslinking
cross-linked
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-5268-02
storage condition
(20% ethanol)
parameter
150 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 6% agarose
matrix active group
Nickel
particle size
~34 μm
cleaning in place
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni²⁺ stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein / mL medium)
separation technique
affinity
storage temp.
2-30°C
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General description
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Features and Benefits
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Preparation Note
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
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Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocols
This page shows how to purify protein A-tagged proteins using Cytiva products.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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