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About This Item
form
resin
crosslinking
cross-linked
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-5268-02
storage condition
(20% ethanol)
parameter
150 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 6% agarose
matrix active group
Nickel
particle size
~34 μm
cleaning in place
2-14(Ni2+ stripped medium.)
operating pH
3-12(Ni²⁺ stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein / mL medium)
separation technique
affinity
storage temp.
2-30°C
General description
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Features and Benefits
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Preparation Note
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
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Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Protocols
This page shows how to purify protein A-tagged proteins using Cytiva products.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Articles
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-5268-02 | 04061837622090 |